flag tag 20543 1 ap proteintech ip Search Results


anti β actin  (Proteintech)
96
Proteintech anti β actin
Anti β Actin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+tag+20543+1+ap+proteintech+ip/bio_rxiv__2021__05__24__445396-219-29-30?v=Proteintech
Average 96 stars, based on 1 article reviews
anti β actin - by Bioz Stars, 2026-08
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rabbit anti flag antibody  (Proteintech)
96
Proteintech rabbit anti flag antibody
Rabbit Anti Flag Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
rabbit anti flag antibody - by Bioz Stars, 2026-08
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flag 20543 1 ap  (Proteintech)
97
Proteintech flag 20543 1 ap
Flag 20543 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+tag+20543+1+ap+proteintech+ip/pm40285620-337-14-19?v=Proteintech
Average 97 stars, based on 1 article reviews
flag 20543 1 ap - by Bioz Stars, 2026-08
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antibodies against flag  (Proteintech)
96
Proteintech antibodies against flag
USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with <t>FLAG-USP25</t> and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with <t>anti-FLAG</t> (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.
Antibodies Against Flag, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+tag+20543+1+ap+proteintech+ip/pmc13015209-54-0-60?v=Proteintech
Average 96 stars, based on 1 article reviews
antibodies against flag - by Bioz Stars, 2026-08
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anti camk2d  (Proteintech)
93
Proteintech anti camk2d
USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with <t>FLAG-USP25</t> and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with <t>anti-FLAG</t> (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.
Anti Camk2d, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+tag+20543+1+ap+proteintech+ip/pmc11186043-217-48-51?v=Proteintech
Average 93 stars, based on 1 article reviews
anti camk2d - by Bioz Stars, 2026-08
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flag tag  (OriGene)
93
OriGene flag tag
USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with <t>FLAG-USP25</t> and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with <t>anti-FLAG</t> (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.
Flag Tag, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+tag+20543+1+ap+proteintech+ip/pmc10444971-58-35-27?v=OriGene
Average 93 stars, based on 1 article reviews
flag tag - by Bioz Stars, 2026-08
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flag  (Proteintech)
96
Proteintech flag
USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with <t>FLAG-USP25</t> and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with <t>anti-FLAG</t> (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.
Flag, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+tag+20543+1+ap+proteintech+ip/pm38388430-67-25-31?v=Proteintech
Average 96 stars, based on 1 article reviews
flag - by Bioz Stars, 2026-08
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asc  (Proteintech)
96
Proteintech asc
USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with <t>FLAG-USP25</t> and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with <t>anti-FLAG</t> (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.
Asc, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+tag+20543+1+ap+proteintech+ip/ppr0501657-102-27-29?v=Proteintech
Average 96 stars, based on 1 article reviews
asc - by Bioz Stars, 2026-08
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rabbit 20543 1 ap  (Proteintech)
96
Proteintech rabbit 20543 1 ap
USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with <t>FLAG-USP25</t> and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with <t>anti-FLAG</t> (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.
Rabbit 20543 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+tag+20543+1+ap+proteintech+ip/pm37120609-194-26-25?v=Proteintech
Average 96 stars, based on 1 article reviews
rabbit 20543 1 ap - by Bioz Stars, 2026-08
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anti flag antibody  (Proteintech)
93
Proteintech anti flag antibody
USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with <t>FLAG-USP25</t> and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with <t>anti-FLAG</t> (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.
Anti Flag Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+tag+20543+1+ap+proteintech+ip/pmc12959842-240-51-70?v=Proteintech
Average 93 stars, based on 1 article reviews
anti flag antibody - by Bioz Stars, 2026-08
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antiflag primary antibody  (Proteintech)
97
Proteintech antiflag primary antibody
USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with <t>FLAG-USP25</t> and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with <t>anti-FLAG</t> (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.
Antiflag Primary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+tag+20543+1+ap+proteintech+ip/10__1016_slash_j__bmc__2020__115772-129-0-3?v=Proteintech
Average 97 stars, based on 1 article reviews
antiflag primary antibody - by Bioz Stars, 2026-08
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bax  (Proteintech)
96
Proteintech bax
USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with <t>FLAG-USP25</t> and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with <t>anti-FLAG</t> (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.
Bax, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+tag+20543+1+ap+proteintech+ip/pmc12394631-75-22-59?v=Proteintech
Average 96 stars, based on 1 article reviews
bax - by Bioz Stars, 2026-08
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Image Search Results


USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with FLAG-USP25 and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with anti-FLAG (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.

Journal: eBioMedicine

Article Title: USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses

doi: 10.1016/j.ebiom.2026.106213

Figure Lengend Snippet: USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with FLAG-USP25 and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with anti-FLAG (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.

Article Snippet: Antibodies against FLAG (Cat#: 20543-1-AP, 1: 1000, RRID: AB_11232216 ), MYC (Cat#: 60003-2-Ig, 1: 1000, RRID: AB_2734122 ), HA (Cat#: 51064-2-AP, 1: 1000, RRID: AB_11042321 ), CD36 (Cat#: 18836-1-AP, 1:1000, RRID: AB_10597244 ), ASK1 (Cat#: 28201-1-AP, 1: 1000, RRID: AB_2782957 ), MLKL (Cat#: 66675-1-Ig, 1:1000, RRID: AB_2882029 ), and p-ASK1 (Cat#: 28846-1-AP, 1: 1000, RRID: AB_2881220 ) were purchased from Proteintech (Wuhan, China).

Techniques: Binding Assay, Molecular Weight, Mass Spectrometry, Immunoprecipitation, Western Blot, Negative Control, Transfection, Lysis, Immunofluorescence